The importance of growing cells in a condition resembling that of the native state - rather than the "fried egg" phenotype of cells cultured in flasks - is increasingly understood. For example, we have observed changes in the electrophysiology of cancer cells grown in a 3D matrix when compared to 2D culture (Biochimica et Biophysica Acta).

 

To this end we have developed a new system of 3D cell culture, using dielectrophoresis to concentrate cells into 100um-diameter hemispheres, which are then held in place by hydrogel. This system has developed from early origins (Electrophoresis) to a well-characterised method which has been shown to affect the response of adherent cells, but not suspension cells, to drug treatment - exactly as would be anticipated (Scientific Reports)