Description of the Technology

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High-Throughput, Low-Loss, Low-Cost and Label-Free Cell Separation using Electrophysiology Activated Cell Enrichment. SA Faraghat, KF Hoettges, MK Steinbach, D van der Veen, WJ Brackenbury, EA Henslee, FH Labeed and MP Hughes, Proceedings of the National Academy of Sciences of the USA 2017 114 4591-4596

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Ten–Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis. KF Hoettges, EA Henslee, RM Torcal Serrano, RI Jabr, RG Abdallat, AD Beale, A Waheed, P Camelliti, CH Fry, DR van der Veen, FH Labeed, MP Hughes. Scientific Reports 2019 9 19153

 

 

Cancer

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Dielectrophoretic analysis of treated cancer cells for rapid assessment of treatment efficacy. S Mahabadi, FH Labeed, MP Hughes. Electrophoresis 2018 39 1104-1110

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Human oral cancer cells with increasing tumorigenic abilities exhibit higher effective membrane capacitance. X Liang, KA Graham, AC Johannessen, DE Costea, FH Labeed. Integrative Biology 2014 6 545-554

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A dielectrophoretic method of discrimination between normal oral epithelium, and oral and oropharyngeal cancer in a clinical setting. KA Graham, HJ Mulhall, FH Labeed, MP Lewis, KF Hoettges, N Kalavrezos, J McCaul, C Liew, S Porter, S Fedele, and MP Hughes. Analyst 2015 140 5198-5204

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Epithelial cancer cells exhibit different electrical properties when cultured in 2D and 3D environments. HJ Mulhall, MP Hughes, B Kazmi, MP Lewis, FH Labeed. Biochimica et Biophysica Acta 2013 1830 5136-5141

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Cancer, pre-cancer and normal oral cells distinguished by dielectrophoresis. HJ Mulhall, FH Labeed, B Kazmi, DE Costea, MP Hughes, MP Lewis. Analytical and Bioanalytical Chemistry 2011 401 2455-2463

 

 

Red blood cells

 

Detecting Circadian Rhythms in Human Red Blood Cells by Dielectrophoresis. D Beale, FH Labeed, SJ Kitcatt, JS O’Neill. Methods in Molecular Biology 2022 2482 255-264.

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Cytoplasmic anion/cation imbalances applied across the membrane capacitance may form a significant component of the resting membrane potential of red blood cells. MP Hughes, CH Fry, FH Labeed. Scientific Reports 2022 12 15005

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Vm-related extracellular potentials observed in red blood cells. MP Hughes, EJ Kruchek, AD Beale, SJ Kitcatt, S Qureshi, ZP Trott, O Charbonnel, PA Agbaje, EA Henslee, RA Dorey, R Lewis, FH Labeed. Scientific Reports 2021 11 19446

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78. Rapid, low-cost dielectrophoretic diagnosis of bladder cancer in a clinical setting. R Hoque, H Mostafid, MP Hughes. IEEE Journal of Translational Engineering in Health and Medicine 2020 8 4300405 (1-5)

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Casein Kinase 1 underlies temperature compensation of circadian rhythms in human red blood cells. AD Beale, E Kruchek, SJ Kitcatt, EA Henslee, JSW Parry, G Braun, R Jabr, M von Schantz, JS O’Neill, FH Labeed. Journal of Biological Rhythms 2019 34 144-153

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Rhythmic K+ transport regulates the circadian clock in human red blood cells. EA Henslee, P Crosby, SJ Kitcatt, JSW Parry, A Bernardini, RG Abdallat, G Braun, HO Fatoyinbo, EJ Harrison, KF Hoettges, AB Reddy, RS Edgar, RI Jabr, M von Schantz, JS O’Neill, FH Labeed. Nature Communications 2017 8 1978.

 

 

Cartilage

 

Transcriptome-based screening of ion channels and transporters in a migratory chondroprogenitor cell line isolated from late-stage osteoarthritic cartilage. C Matta, R Lewis, C Fellows, G Diszhazi, J Almassy, N Miosge, J Dixon, MC Uribe, S May, S Poliska, R Barrett-Jolley, E Henslee, FH Labeed, MP Hughes, A Mobasheri. Journal of Cellular Physiology 2021 236 7421-7439

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Dielectrophoresis as a Tool to Reveal the Potential Role of Ion Channels and Early Electrophysiological Changes in Osteoarthritis. R Abdallat, EJ Kruchek, C Matta, R Lewis, FH Labeed. Micromachines 2021 12 949

 

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Other cells

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In Vitro characterization of murine pre-adipose nucleated cells reveals electrophysiological cycles associated with biological clocks. C Martin, JD Johnston, EA Henslee, DR can der Veen, FH Labeed. Electrophoresis 2022 43 1337-1346

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The platelet electrome: evidence for a role in regulation of function and surface interaction . MP Hughes, EJ Kruchek, JM Gibbins, OV Griffiths, B Abdal, FH Labeed. Bioelectricity 2022 4 153-159

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Characterization of human skeletal stem and bone cell populations using dielectrophoresis. A Ismail, MP Hughes, HJ Mulhall, ROC Oreffo, FH Labeed. Journal of Tissue Engineering and Regenerative Medicine 2015 9 162-168

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Cytoplasm resistivity of mammalian atrial myocardium determined by dielectrophoresis and impedance methods. CH Fry, SC Salvage, A Manazza, E Dupont, FH Labeed, MP Hughes, RI Jabr. Biophysical Journal 2012 103 2287-2294

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Biophysical characteristics reveal neural stem cell differentiation potential. FH Labeed, JT Lu, HJ Mulhall, SA Marachenko, KF Hoettges, LC Estrada, AP Lee, MP Hughes, L Flanagan. PLoS One 2011 6e25458

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Rapid determination of antibiotic resistance in E. coli using dielectrophoresis. K. F. Hoettges, J. W. Dale, M. P. Hughes. Physics in Medicine and Biology 2007 52 6001-6009 

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Methods

Effects of cell detachment methods on the dielectric properties of adherent and suspension cells. S Mahabadi, FH Labeed, MP Hughes. Electrophoresis 2015 36 1493–1498

Accurate quantification of apoptosis progression and toxicity using a dielectrophoretic approach. EA Henslee, RM Torcal Serrano, FH Labeed, RI Jabr, CH Fry, MP Hughes and KF Hoettges. Analyst 2016 141 4608-4615

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Apoptosis Progression Studied using Parallel Dielectrophoresis Electrophysiological Analysis and Flow Cytometry. HJ Mulhall, A Cardnell, KF Hoettges, FH Labeed and MP Hughes. Integrative Biology 2015 7 1396-1401

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Real-time cell electrophysiology using a multi-channel dielectrophoretic-dot microelectrode array. HO Fatoyinbo, NA Kadri DH Gould, KF Hoettges, FH Labeed. Electrophoresis 2011 32 2541-2549

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Dielectrophoretic analysis of changes in cytoplasmic ion levels due to ion channel blocker action reveals underlying differences drug sensitive and multidrug resistant leukaemic cells.  L Duncan, H Shelmerdine, Y. Hübner, HM Coley, MP Hughes, FH Labeed. Physics in Medicine and Biology, 2008 53 N1-N7

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Non-biological particles

 

Rapid determination of nanowire electrical properties using a dielectrophoresis-well based system. M Constantinou, KF Hoettges, S Krylyuk, A Davydov, GP Rigas, MP Hughes, M Shkunov. Applied Physics Letters 2017 110 133103 (1-5)

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Dielectrophoretic sample preparation for environmental monitoring of microorganisms: soil particle removal. HO Fatoyinbo, MC McDonnell, MP Hughes. Biomicrofluidics 2014 8 044115 (1-10)

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Development of the DEP-Well

 

Efficient dielectrophoretic cell enrichment using a DEP-well based system. MA Abdul Razak, KF Hoettges, HO Fatoyinbo, FH Labeed, MP Hughes. Biomicrofluidics 2013 7, 064110 (1-10)

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Rapid, automated measurement of dielectrophoretic forces using DEP-activated microwells. LM Broche, KF Hoettges, SL Ogin, GEN Kass, MP Hughes. Electrophoresis 2011 32 2393-2399

 

Dielectrophoresis-activated multiwell Plate for label-free high-throughput Drug Assessment. KF Hoettges Y Hübner, LM Broche, SL Ogin, GEN Kass, MP Hughes. Analytical Chemistry 2008 80 2063-2068

 

Parallel measurements of drug actions on Erythrocytes by dielectrophoresis, using a three-dimensional electrode design. Y Hübner, KF Hoettges, GEN Kass, SL Ogin, MP Hughes. IEE Proceedings on Bionanotechnology 2005 4 21-25

 

A high-throughput 3D composite dielectrophoretic separator. HO Fatoyinbo, D Kamchis, R Whattingham, SL Ogin, MP Hughes. IEEE Transactions on Biomedical Engineering 2005 52 1347-1349